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“If the staining technique is done improperly, many of the quick Romanowsky-type stains will not give the needed tinctorial differences between mature RBCs (orange-red) and polychromatophils (bluish-pink). Typically, poor staining with the various quick stains results in all RBCs having a bluish or muddy tint.”

-Fred L. Metgzer Jr., DVM, DABVP, and Alan Rebar, DVM, PhD, DACVP

From Three-minute peripheral blood film evaluation: Preparing the film