News|Articles|October 1, 2025

Central venous catheter placement and maintenance

This step-by-step guide outlines safe placement, maintenance, and removal of central venous catheters, as well as supplies, indications, and contraindications to reduce complications in patients.

Central venous catheters (CVCs) are critical tools in veterinary care, providing reliable vascular access for fluid therapy, medication delivery, parenteral nutrition, and repeated blood sampling in critically ill patients. While their use can be essential to patient survival, introducing a catheter into central circulation carries greater risks than peripheral catheterization. Potential complications include catheter-related bloodstream infections, thrombotic events, and mechanical issues such as malposition or vessel injury. To minimize these risks, strict aseptic technique and meticulous catheter management are required throughout placement, maintenance, and removal.

This review outlines the key indications, contraindications, necessary supplies, and a step-by-step approach to safe and effective CVC use in veterinary medicine.

Supplies

Some items may be included with the central line kit:

  • Clippers
  • 0.2 mL idocaine for local block
  • Tape measure
  • Chlorhexidine scrub-soaked gauze
  • Alcohol-soaked gauze
  • Sterile gauze
  • Sterile gloves
  • Appropriately sized catheter-gauge and length
  • Fenestrated drape
  • Sterile 0.9% NaCl flushes x number of lumens
  • Suture material
  • Needle drivers with scissors
  • Male adapter
  • Cast padding
  • Vetwrap
  • +/- Sterile gown
  • +/- Sedation with appropriate monitoring devices

Placement preparation

Step 1: Perform proper hand hygiene.

Step 2: Don nonsterile gloves.

Step 3: Place the animal in the preferred recumbency.

  • The restrainer wears nonsterile gloves.
  • Measure the distance from the intended insertion site to the desired placement.
    • Jugular vein:
      • This is the insertion site to the thoracic inlet.
      • The tip of the catheter should lie within the thoracic inlet, just cranial to the right atrium.
    • Lateral/medial saphenous veins:
      • Insertion site to the last rib
      • Caudal vena cava

Step 4: Perform a wide clip of fur using a clean #40 clipper blade, centered on the intended venipuncture site.

  • Ensure the area is large enough so no part of the catheter, including suture, will be within the patient’s fur.
  • Avoid unhealthy skin.

Step 5: Clean the area with gauze soaked in chlorhexidine scrub

  • Be thorough but gentle when scrubbing the area, as abraded skin will support colonization of pathogenic bacteria.
  • Do not clean the same place twice with the same gauze.
  • Clean residual scrub from skin with alcohol to prevent dermatitis.
  • Do this procedure again, alternating between scrub and alcohol 2 times.
  • Do not touch the insertion site after preparation.

Sterile technique

Step 1: Open supplies using sterile technique.

Step 2: Don sterile gloves +/- sterile gown.

Step 3: Using sterile technique, flush with 0.9% sodium chloride solution (NaCl) and cap all ports prior to insertion.

Step 4: Drape the patient to prevent inadvertent contamination of the catheter (Figure 1).

  • Palpate the vessel while the restrainer occludes the vessel.
  • Infiltration of a local anesthetic at the intended insertion site is recommended for nonsedated animals.

Step 5: Insert introducing needle or short over-the-needle-catheter bevel up, going toward the direction of the heart.

Step 6: When blood is observed flashing back into the needle, if using a short over-the-needle catheter, advance the catheter and then remove the stylet before feeding the guide wire.

  • The distal end of the wire has a flexible J-tip to prevent puncturing the vessel.
  • Feed the guide wire approximately two-thirds to three-quarters of its length into the vessel.
  • Place sterile gauze over the insertion site with a slight amount of pressure while removing the insertion needle or short over-the-needle-catheter to prevent blood loss
  • Never let go of the wire.

Step 7: Thread the vessel dilator over the wire.

Step 8: Grasp the dilator near the distal tip and advance it into the vessel using a forward, twisting motion.

  • The skin entry site may need to be enlarged using a scalpel blade (RVT or DVM only).
    • Minimize cutdowns; larger cutdowns increase the risk of bacterial colonization.

Step 9: Remove the vessel dilator while still holding the wire.

Step 10: Place sterile gauze over the insertion site with a slight amount of pressure while removing the vessel dilator to prevent blood loss.

Step 11: Remove the cap from the distal (brown) port of the catheter and ensure it is not clamped.

Step 12: Thread the multilumen catheter over the wire until the proximal end of the wire protrudes from the port.

Step 13: While holding the distal end of the guide wire, advance the catheter into the vessel until the desired distance is achieved (as determined by the previous measurement).

Step 14: While holding the catheter in place, remove the guide wire.

Step 15: Attach a syringe of sterile 0.9% NaCl to the port and aspirate blood to ensure proper placement and remove any air bubbles.

  • Flush open port, clamp and then place the infusion cap on the end.
  • Repeat with all ports.

Step 16: Suture the catheter to the skin using the plastic clamps with sterile needle drivers (Figure 2).

  • All single or multilumen CVCs should be sutured in.
  • If the catheter is too long, place the provided spacers over the exposed catheter before suturing.

Step 17: Take a lateral placement radiograph to ensure proper placement of the tip of the catheter (Figures 3a, 3b, and 4).


Step 18: Cover the site with a small nonadherent pad and small Tegaderm (avoid ointments).

  • The bandage should be firm, but never tight.
    • Peripheral catheters:
      • Wrap with cast padding, stretch gauze, and vet wrap.
    • Jugular catheters (Figure 5):
      • Wrap with cast padding, followed by vet wrap.
      • Do not use stretch gauze.
    • Secure lumens with tape so they don’t get caught on anything.

Maintenance

Step 1: Wipe injection ports with alcohol before using.

Step 2: A 250-mL bag should be labeled when first punctured "For this patient's use only"; it expires in 72 hours (please refer to hospital policies regarding sampling).

Step 3: Flush CVC with sterile 0.9% NaCl every 6 hours.

  • Unwrap bandage and visualize insertion site every 24 hours (change bandage).
  • Check for signs of phlebitis/infection/dislodgment, redness, swelling, heat or discharge and notify the doctor of any abnormalities.
  • If a portion of the catheter becomes visible and has pulled out from the insertion site:
    • Aseptically clean the area with 2% chlorhexidine solution.
    • Place spacers on top of the exposed catheter.
    • Do not reinsert the catheter back into the vessel.

Step 4: When a patient is disconnected from intravenous (IV) fluids, a new male adapter should be used to cover the CVC hub and cap the fluid line with the other end of the male adapter.

Step 5: There is no need to remove the CVC unless signs of phlebitis/infection/dislodgement occur.

Step 6: Evaluate the need for a catheter and remove it as soon as it is no longer necessary to decrease the chances of bloodstream infection.

Removing the catheter

Step 1: Using 2% chlorhexidine solution, followed by sterile 0.9% NaCl, clean a circular area 2 inches in diameter around the insertion site, beginning at the insertion site and moving outward. This reduces microbial skin contaminants from migrating into the site.

Step 2: Withdraw CVC slowly in 1 continuous motion.

  • Stop if resistance is met: The catheter may be knotted or lodged in the vessel. Secure the CVC, cover the site, and report to the physician.

Step 3: Place pressure on the site with clean gauze dressing until hemostasis is achieved; 1 to 5 minutes is suggested.

Step 4: Apply clean gauze dressing, cover with a transparent film dressing, wrap with cast padding, then use vet wrap to prevent air emboli.

Step 5: Leave the bandage in place for about an hour.

Indications

  • Long-term IV access
  • Multiple fluid lines/IV medications
  • Serial blood sampling
  • Lack of peripheral access

Contraindications

  • Bleeding disorders
  • Coagulopathies/thrombocytopenia
  • Increased intracranial pressure: potential for decreased venous drainage during placement

Jenn Bench, RVT, VTS (ECC), T-CCFP, discovered her passion for veterinary emergency and critical care while completing her externship at a specialty emergency and critical care hospital during her time at the Bel-Rea Institute of Animal Technology in Aurora, Colorado. After earning her Registered Veterinary Technician (RVT) credentials, she pursued and achieved her Veterinary Technician Specialty (VTS) in emergency and critical care.

In her current role as the regional director of education and development for ACCESS Specialty Animal Hospitals, a Thrive Pet Healthcare facility, Bench is dedicated to mentoring and training support staff, veterinary technicians, and doctors. She finds great fulfillment in education, with a particular passion for training CPR. Jenn is also a certified compassion fatigue professional, advocating for mental wellness and resilience in veterinary medicine.

Newsletter

From exam room tips to practice management insights, get trusted veterinary news delivered straight to your inbox—subscribe to dvm360.