• DVM360_Conference_Charlotte,NC_banner
  • ACVCACVC
  • DVM 360
  • Fetch DVM 360Fetch DVM 360
DVM 360
dvm360 | Veterinary News, Veterinarian Insights, Medicine, Pet Care
dvm360 | Veterinary News, Veterinarian Insights, Medicine, Pet Care
By Role
AssociatesOwnersPractice ManagerStudentsTechnicians
Subscriptions
dvm360 Newsletterdvm360 Magazine
News
All News
Association
Breaking News
Conference Coverage
Education
Equine
FDA
Law & Ethics
Market Trends
Medical
Politics
Products
Recalls
Regulatory
Digital Media
dvm360 LIVE!™
Expert Interviews
The Vet Blast Podcast
Medical World News
Pet Connections
The Dilemma Live
Vet Perspectives™
Weekly Newscast
dvm360 Insights™
Publications
All Publications
dvm360
Firstline
Supplements
Vetted
Clinical
All Clinical
Anesthesia
Animal Welfare
Behavior
Cardiology
CBD in Pets
Dentistry
Dermatology
Diabetes
Emergency & Critical Care
Endocrinology
Equine Medicine
Exotic Animal Medicine
Feline Medicine
Gastroenterology
Imaging
Infectious Diseases
Integrative Medicine
Nutrition
Oncology
Ophthalmology
Orthopedics
Pain Management
Parasitology
Pharmacy
Surgery
Toxicology
Urology & Nephrology
Virtual Care
Business
All Business
Business & Personal Finance
Hospital Design
Personnel Management
Practice Finances
Practice Operations
Wellbeing & Lifestyle
Continuing Education
Conferences
Conference Listing
Conference Proceedings
Resources
CBD in Pets
CE Requirements by State
Contests
Veterinary Heroes
Partners
Spotlight Series
Team Meeting in a Box
Toolkit
Top Recommended Veterinary Products
Vet to Vet
  • Contact Us
  • Fetch DVM360 Conference
  • Terms and Conditions
  • Privacy
  • Do Not Sell My Information
  • About Us

© 2023 MJH Life Sciences and dvm360 | Veterinary News, Veterinarian Insights, Medicine, Pet Care. All rights reserved.

Advertisement
By Role
  • Associates
  • Owners
  • Practice Manager
  • Students
  • Technicians
Subscriptions
  • dvm360 Newsletter
  • dvm360 Magazine
  • Contact Us
  • Fetch DVM360 Conference
  • Terms and Conditions
  • Privacy
  • Do Not Sell My Information
  • About Us
  • MJHLS Brand Logo

© 2023 MJH Life Sciences™ and dvm360 | Veterinary News, Veterinarian Insights, Medicine, Pet Care. All rights reserved.

Basic approach to evaluating solid tissue tumor cytology (Proceedings)

August 1, 2011
Christine Olver, DVM, PhD, DACVP

An epidermal, dermal, or subcutaneous mass lesion that is easily accessible to aspiration cytology

Lump or bump

An epidermal, dermal, or subcutaneous mass lesion that is easily accessible to aspiration cytology

Goal of aspiration cytology

• To direct next step of therapy or diagnostics (at the minimum)

• To definitively diagnose the cause of the lesion

• To advise owners of most probable process

What makes it a lump?

• Expansion of tissue

• Tissue fluid

• Infiltration cells

• Overgrowth of resident cells

Overall question

• What is the process that is causing this expansion?

• Subquestions:

     o What is the treatment?

     o What is the prognosis?

Advertisement

Obtaining the sample

• Fine needle biopsy (aspirates)

     o Preferred method for masses

• Impression smear

• Scraping

• Swab

Staining

• Romanowsky stains

     o Metachromatic reaction

• Romanowsky-like stains (Diff-Quik)

     o Mast cells may not stain

What is the general process?

• Tissue fluid

• Cells

     o Inflammatory

     o Neoplastic

Fluid/cysts

• Keratin containing cysts

• Hematoma

Inflammation

• Identify leukocytes

• Search for and identify etiologic agents

What is the identity of the tissue on the preparation?

• Discrete: no cell to cell association

• Epithelial: tight clusters of round-ish cells

• Mesenchymal: loose aggregates of fusiform cells

Interpretation of samples

• Inflammatory versus neoplastic

• Neoplastic

     o Histogenesis: from what tissue has it arisen?

     o Benign v. malignant

Neoplasia histogenesis

• Discrete

• Epithelial

• Mesenchymal

Discrete neoplasms

• Lymphoma

• Mast cell tumor

• Histiocytoma

• Transmissable venereal tumor

• Malignant histiocytosis

• Plasmacytomas

• +/- melanoma

Mesenchymal neoplasms

• Dogma: poorly exfoliating

• Groups of cells appear off a "stalk"

• Many cells are individualized

• Fusiform shape

Epithelial neoplasms

• Sheets or clumps

• Acinar or ductal structures

• Round to polygonal versus fusiform/spindle

Criteria of malignancy

• Anisocytosis/anisokaryosis

• Pleomorphism

• Macrokaryosis (nuclei >10 microns)

• Increased nuclear to cytoplasmic ratio

• Multinucleation

• Abnormal mitoses

• Nuclear molding

• Macronucleoli

• Angular nucleoli

Specific lesions

• Lipoma: What if you had something then you have nothing?

• Sebaceous gland adenoma

• Masses on the south end (of a dog going north)

     o Two choices: apocrine gland adenocarcinoma or perianal gland tumor

• Histiocytoma

• Mast cell tumor

• Plasma cell tumor

• Epidermal or adnexal epithelial neoplasia

• Keratin containing cyst

Related Content:

Medical
Not-so ordinary ER cases you should know
Not-so ordinary ER cases you should know
An update on copper concerns in pet foods
An update on copper concerns in pet foods
Traditional and holistic treatments for giardia
Traditional and holistic treatments for giardia

Advertisement

Latest News

An update on copper concerns in pet foods

Dental hacks to make every case more manageable

Q&A with a keynote: Walter Brown, RVTg, VTS, ECC

News wrap-up: This week’s headlines, plus dvm360® launches its first CE podcast

View More Latest News
Advertisement