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Managing and treating sick neonates (Proceedings)
The goal of neonatal care is to maximize the health and well being of the newborn puppy and kitten.
The goal of neonatal care is to maximize the health and well being of the newborn puppy and kitten. The first few weeks of life are the most perilous. Most deaths occurring during this critical period are a result of a failure to meet the physiological needs of the newborn. Understanding these requirements, early problem recognition, and effective therapeutic plans, will greatly enhance the survival of the sick or marginal youngster.
Death loss: Average puppy and kitten deaths during the first 12 weeks of life approach 11%-34%. Still births or death within the first 24 hours account for 5% of the losses; an additional 5% loss occurs during the neonatal period; and 0%-5% loss in transitional and socialization periods. Infectious diseases are not the most common cause of neonatal or transitional period mortality.
Birth weight/weight gain: Birth weight is the single most important predictor of neonatal survival. Those neonates that are < 25% of the litter average weight are at particularly high risk for hypoglycemia, hypothermia, hypoxia, bacterial septicemia and pneumonia. Close observation and careful monitoring are paramount to their survival chances. Monitoring weight gain is a good indicator of health status. Reported criteria for adequate weight gain have been reported during the neonatal period include; the puppies should double their weight in 10 days; the puppies should gain 5-10%/day; the puppies should gain 2 - 4 g/kg of the expected adult weight/day. Nursing kittens should also double their weight in 10 days; normal kittens gain 10 - 15 G/day; and the kittens should weigh 1 pound/month for the first 4 months. Formula fed neonates grow at significantly slower rate despite the identical caloric intake.
Health monitoring: Signs of healthy vigorous neonates include; adequate weight gain, strong activated sleep patterns, firm muscle tone and strength, and not crying. Crying for over 15 minutes is a signal. Puppies cry when hungry, neglected (separated or culled), in pain, and especially when cold.
Hypothermia: Thermoregulation is problematic in the neonate. Chilling is always a major threat to the survival of the neonate. The shivering reflex and peripheral vasoconstriction response are not fully developed until at least 1 week. Their relatively large surface area, plus the lack of insulating fat, promotes rapid heat loss by conduction, convection, radiation, and evaporation. The vulnerable young must relay on warmth of the dam and litter and environment to maintain an adequate body temperature. Mothers who refuse to "gather" the young or lick the young excessively, or "cull" one or two of the young, places these individuals at high risk for hypothermia. Hypothermia is a common cause of death in the newborn and is part of a viscous down spiraling cascade of events. As the rectal temperature reaches below 94° F the neonate suckling becomes weak and ineffectual. The intestines become hypomotile and the heart rate increases. Below 85° F there is gastrointestinal stasis with bacterium, a decrease in heart rate and hypoglycemia. Once below 70° F, the neonate is motionless and appears appear dead. An occasional chest wall movement may be seen, but the heart rate is 40-60 b/min and is non-palpable. Environmental temperature can be critical as a healthy newborn can only maintain a body temperature 12°F > than that of the surrounding environment.
Hypothermic patients should be re-warmed slowly (1-2 hours) to a temperature of 98°- 99°F. Warming increases the respiratory and heart rates; increases effectual nursing and swallowing reflex; increases visceral movements; and mobilizes glycogen stores. This warming process is essential prior to attempted feeding. Maintain the neonate in a draft-free environment. Re-warming is best accomplished with a human neonatal incubator. A thermometer should be used to measure ambient temperature. Focal heat sources such as circulating hot water blankets, warmed rice bags and hot water bottles insulated with towels may also be used. Whenever a focal heat source is used, a temperature gradient should be created to allow the neonate to either move to or away from the heat source. Overheating is rarely a problem but panting with hyperemic membranes and skin is a clue. Heat lamps and electric heating pads are not recommended because of increased the risks of burns and overheating. Remember to turn the patient every 20 minutes while taking the temperature.
Hypoxia: Hypoxia is a common sequel to birthing. Many newborns would benefit from short term supplemental oxygen therapy and respiratory stimulants such as doxapram HCl. Those neonates with pneumonia or sepsis often require supplemental oxygen via an oxygen tent, oxygen cage, nasal tube or face mask (short-term only). Arterial blood gases are nearly impossible to collect and unavailable to most practices. However, a pulse- oxymeter can sometimes be placed on the hairless skin of the ventral abdomen. Unfortunately an accurate reading requires adequate circulation not often present in a shocky neonate. The normal oxygen saturation is > 90%.
Dehydration: Dehydration is always a concern with a sick puppy or kitten. It is no surprise they are extremely susceptible to dehydration. They are >70% water with a large surface area is covered with non-cornified skin combined with an inability to concentrate their urine. Hydration status can not be accurately assessed with skin turgor. Estimates of the degree of dehydration must be determined by dryness of the mucus membranes and eyes, plus the urine specific gravity. Accurate body weight and urine specific gravity can be used to evaluate the rehydration efforts. Re-hydration fluids may be administered to the neonate orally via stomach tube, subcutaneously, intravenously, interosseous, or rectally depending on the severity of the problem and resources available. Regardless of the route, fluids should be pre-warmed to 95°F – 98.6°F. Maintenance fluid requirements in neonates are about 60 -100 ml/kg/day.
For oral stomach tube fluid administrations use a 5 FR – 8 FR infant feeding tube. Measure from the tip of the nose to the last rib and mark the tube. The tube is filled with fluid to prevent the introduce air into the stomach. Pass the tube down the left side of the mouth as the puppy cries (exhales). After delivery of the fluid, pinch the tube prior to withdrawal and withdraw it quickly to minimize aspiration. Aspiration pneumonia is a fatal consequence of improper placement of the feeding tube. Normal stomach volume is approximately 50 ml/kg. Oral fluids and nutrition are contraindicated in hypothermic neonates because gastrointestinal motility.
Subcutaneous fluids may be given in the interscapular space similar to a mature animal. However absorption is delayed with hypothermia. The jugular vein may be catheterized for intravenous fluids using a 24-ga over-the-needle catheter. The interosseous route is best method of fluid administration in the small neonate. A 22-ga spinal needle or standard 20 – 22-ga needle placed intramedullary in the femoral cortex via the trochanteric fossa is an excellent way to administer fluids or blood. The site should be clipped, aseptically prepared and blocked. The needle is rotated back and forth as it is pushed into and firmly seated in the cortex. Pain is associated with cold fluids or fluids given to rapidly. The initial fluid of choice is warmed 50:50 solution of lactated ringers and 5% dextrose or LRS. Potassium supplementation may be necessary. Monitoring the patient's weight, cardiopulmonary status, mucus membranes, and urine specific gravity can be used to evaluate the response to fluid therapy.
Hypoglycemia: Next to hypothermia, hypoglycemia is one of the most common and serious problems seen in the neonates, especially the toy breeds. Normal blood glucose for the neonate is 90-140 mg%. Maintenance of normal blood glucose requires several interrelated factors of digestive absorption, liver and muscle glycogenolysis, and liver gluconeogenesis. A fasting puppy can maintain adequate blood glucose for 24 hours initially through glycogenolysis then gluconeogenesis. After that period a precipitous drop occurs. Neonates are prone to hypoglycemia because of increased demands for glucose (in part due to low fat reserves), poor hepatic and muscle glycogen reserves and reduced precursors for gluconeogenesis. Hypoglycemia is often secondary or a consequence of some other disease process (i.e. sepsis). Because it is such a common squeal to most problems empirical treatment of hypoglycemia is recommended for all sick neonates. Clinical signs of hypoglycemia include; visual problems, vertigo, in coordination, muscle tremors, seizures, lethargy, depression, collapse, coma, death. Unfortunately these symptoms can also be associated with numerous other common and uncommon neonatal illnesses. The diagnosis of hypoglycemia is based on the clinical signs, blood glucose, and the response to dextrose therapy. Therapy options include 10% Dextrose dosed at 1-2 ml/kg given slowly intravenously or 1-2 ml/kg 10% dextrose given orally via stomach tube every 15 minutes until normoglycemic. Owners can be instructed to give oral Nutrical, Nutri-Drops, honey, Kayro syrup but only if the patient is conscience. Once stabilized initiate L-carnitine 50 mg/kg PO BID which increases the livers ability to convert fat into glucose. L-carnitine can be used as a preventative in at risk patients.
Nutrition: Always one of the first things an owner is concerned about with a sick puppy or kitten, however it is one of the last items to consider in managing a sick neonate. Once the hypothermia, hypoxia, hypoglycemia and dehydration have been addressed, and then consider nutritional supplementation. Tube feeding with a commercial milk replacement formula initially diluted 50% with LRS is the next step in patient care of the sick neonate.
Neonatal septicemia: Life threatening sepsis occurs when a bacterial infection overcomes a neonates defense mechanisms. Staphylococcus, Streptococcus, E coli, Klebsiella, Enterobacter, Clostridium, and Salmonella are commonly isolated from septic neonates. Ports of entry include the gastrointestinal tract, respiratory tract infection, urinary tract, skin and the umbilical cord. Several pre-disposing issues are responsible for either a decrease in the host's immunity and/or a break in the physical protection barriers. Those co-existing factors include inadequate colostrum, hypothermia, hypoglycemia, poor nutrition, viral infection, endoparasitism, plus metritis and mastitis in the bitch or queen. The clinical sings of septicemia vary with the severity of the condition. Most findings are not pathognomonic for sepsis and may be overlooked. Occasionally death is par-acute with no symptoms noted. Typically neonates should several of the following; prolonged crying, restlessness, weakness, hypothermia, shock, cyanosis. Petechial hemorrhages may be present. In advanced cased there may be discoloration and/or sloughing of distal extremities i.e., toes, ears, tongue, ear tips and tail. Laboratory findings are supportive of the diagnosis. Initially the hemogram will reveal a neutrophillic leukocytosis with a mild left shift. The marrow reserves are rapidly diminished resulting in a neutropenia. A thrombocytopenia is often present. An associated hypoglycemia is usually present.
A septic neonate should be managed as an emergency. Any commonly associated conditions such as hypothermia, hypoxia, hypoglycemia, shock, and dehydration need to be addressed. Prior to initiating antibacterial therapy, any cultures should be taken for submission. Antibacterial selection is empirical in the neonate as little clinical pharmacokinetic data on the appropriate dosing of antimicrobial agents is lacking. Depending on the specific drug, absorption, re-distribution, protein binding, lipid solubility, hepatic metabolism, renal clearance, and poorly developed blood brain barrier influences the dosage regimen. Potential toxicities are an issue. Because absorption of antibacterial following oral, subcutaneous, or intramuscular administration is unpredictable, the antibacterial agents used in septicemia should be administered IV or interosseous if possible.
Published dosages for selected agents;
Amoxicillin 11-22 mg/kg PO/IV BID; Amoxicillin with clavulanic acid 12-25 mg/kg PO BID;
Cephalexin 10-30 mg/kg PO BID-TID ; Cephazolin 10 mg/kg PO/IV BID;
Ticarcillin with clavulanic acid 15 mg/kg IV/IM BID; Trimethoprim Sulfa 30 mg/kg PO Q24H.
Fading puppy/fading kitten syndrome: Puppies and kittens that slowly waste away then die during the neonatal period often referred to as a "fading puppy " or "fading kitten" syndrome. The pups and kittens are born apparently healthy, but nursing slowly ceases, they fail to gain weight, become weak, thin, and eventually die, for no apparently obviously reason. The syndrome represents a myriad of causative agents. Rule outs should include congenital anomalies, nutritional deficiencies in the mother, traumatic birth injury, maternal neglect, and neonatal isoerythrolysis. Infectious agents such as Brucellosis, low grade bacterial sepsis, viral infections (herpes virus, FIP/FeLV) and toxoplasmosis have been incriminated in the "fading" syndrome. Unfortunately, some fading neonates will not survive. Obtaining complete and accurate necropsies is the most expensive and crucial aspect of identifying the cause of the "fading". A necropsy diagnosis may yield information that will be beneficial to the rest of the litter and future litters. The neonate should be kept refrigerated (never frozen) until a trained pathologist performs necropsy. If the necropsy is performed at the clinic, the procedure should include a thorough examination for congenital anatomic defects. Samples of all major organs should be obtained for histology and culture. Formalin-fixed tissues, along with detailed descriptions of gross lesions and clinical histories, should then be forwarded to veterinary pathologists for microscopic examination. If the primary cause of a fading puppy or kitten syndrome is determined, a concerted effort can be made to eliminate the possible reoccurrence in next breeding.